5th Meeting of Regulating with
RNA in Bacteria & Archaea

March 19-22, 2018 • Seville/Spain

5th Meeting of Regulating with
RNA in Bacteria & Archaea

March 19-22, 2018 • Seville/Spain

Congress details

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Poster sessions

20.03.2018 Please note that the posters from poster session 1 and 2 should be hung by Monday, 19:00 h and need to be removed by Wednesday, 09:00 h.
17:20–19:30
Room
Poster session 1
Andalucia
P 001 Activation of the master virulence regulator PhoP in acidic pH requires the RNA-binding protein CspC in Salmonella
Hubert Salvail, Jeongjoon Choi, Eduardo Groisman (West Haven/US)
P 002 Identification of new type I toxin-antitoxin systems associated with CRISPR arrays in the genome of the human pathogen Clostridium difficile
Olga Soutourina (Orsay, Gif Sur Yvette/FR)
P 003 Early life of mitochondrial RNAs in Trypanosoma brucei
Ruslan Afasizhev, Francois Sement, Takuma Suematsu, Tian Yu (Boston/US)
P 004 PPR polyadenylation factor defines mitochondrial mRNA identity and stability in trypanosomes
Inna Afasizheva, Tian Yu, Michail Mesitov (Boston/US)
P 005 The crystal structure of Hfq from Caulobacter crescentus
Steven Hardwick (Cambridge/GB), Andrew Santiago-Frangos, Jeliazko Jeliazkov, Sarah Woodson (Baltimore/US), Ben Luisi (Cambridge/GB)
P 006 Evolving novel riboswitches through in vivo genetic selection and cell sorting
Jane Liu, Katharine Page, Jeremy Shaffer, Samuel Lin (Claremont/US)
P 007 Computationally Deciphering sRNA-based Regulation in Bacteria
Lourdes Peña-Castillo (St. John's/CA)
P 008 Novel High-throughput methods for sRNA Functional characterization and engineering
Lydia Contreras (Austin/US)
P 009 Specific and global regulations of mRNA stability for E. coli adaptation
Sébastien Nouaille, Sandrine Laguerre, Brice Enjalbert, Laurence Girbal, Muriel Cocaign-Bousquet (Toulouse/FR)
P 010 Genome-wide regulation of mRNA stability and transcription by the Csr system contributes to Escherichia coli adaptation
Laurence Girbal, Manon Morin, Brice Enjalbert, Muriel Cocaign-Bousquet (Toulouse/FR)
P 011 Mixed regulatory circuits between small RNAs and two-component systems
Anaïs Brosse, Gaëlle Demarre, Olivier Espéli (Paris/FR), Anna Korobeinikova (Paris/FR, Pushchino/RU), Susan Gottesman (Bethesda/US), Maude Guillier (Paris/FR)
P 013 Monitoring global RNA secondary structure inside a bacterial pathogen
Jörgen Johansson, Dmitriy Ignatov (Umeå/SE)
P 014 The Shiga toxin promoter of enterohaemorrhagic E. coli transcribes a regulatory small RNA that controls RpoS  
Brandon Sy, Jai Tree (Sydney/AU)
P 015 Small RNA ncRv10243A as a modulator of oxidative stress response in Mycobacterium smegmatis
Artem Grigorov, Arina Mazurova, Elena Salina, Tatyana Azhikina (Moscow/RU)
P 016 ncRv11733, a small RNA of Mycobacterium tuberculosis, in adaptation to intracellular stresses
Elena Salina, Artem Grigorov, Konstantin Majorov, Nadya Logunova, Arseny Kaprelyants, Alexander Apt, Tatyana Azhikina (Moscow/RU)
P 017 RNA-membrane interactions as a potential organizing principle for life
James Saenz, Bartosz Kasprzak, Maren Gnaedig, Tomasz Czerniak (Dresden/DE)
P 018 Structural Understanding of How sRNA-mRNA Interactions are Regulated by a New Class of Bacterial RNA Chaperones
Hyeong Jin Kim, Ross Edwards, Shiyun Peng, Ayat Omar, Andrew MacMillan, Mark Glover (Edmonton/CA)
P 019 sRNAs originate de novo or by recycling vestigial genetic elements in bacteria
Hannah Dutcher, Rahul Raghavan (Portland/US)
P 020 Post-transcriptional regulation of stress-acclimation protein NblA by a conserved small RNA in heterocyst-forming cyanobacteria
Isidro Álvarez-Escribano, Alicia M. Muro-Pastor, Agustín Vioque (Sevilla/ES)
P 021 Two non-coding RNAs in Staphylococcus aureus are involved in glucose metabolism and virulence
Emma Desgranges, Delphine Bronesky (Strasbourg/FR), Patrice François (Geneva/DE), Alejandro Toledo-Arana (Mutilva/ES), Karen Moreau, François Vandenesch (Lyon/FR), Stefano Marzi, Pascale Romby, Isabelle Caldelari (Strasbourg/FR)
P 022 Elucidate RNA turnover in single mycobacterial cells as source of noise and adaptation
Anna Griego, Giulia Manina (Paris/FR)
P 023 Identification of Pseudomonas aeruginosa MiaA dependent tRNA modifications by LC-MS/MS and their impact on virulence
Svenja Grobe, Jonas Krüger, Adrian Kordes, Volkhard Kaever (Hannover/DE), Susanne Häußler (Hannover, Braunschweig/DE)
P 024 Gene network analysis identifies a sRNA regulator of stress survival in Caulobacter crescentus
Matthew Tien, Aretha Fiebig, Sean Cro (Chicago/US)
P 025 A conserved 3'UTR-derived small RNA from the sdh-suc operon regulates different sets of genes in Salmonella and E. coli
Masatoshi Miyakoshi (Akita/JP, Würzburg/DE), Kanako Maki, Yasuhiro Sone (Akita/JP), Jörg Vogel (Würzburg/DE)
P 026 Identification of Pasteurella multocida small RNAs: unravelling the regulatory network
Marianne Mégroz, Emily Gulliver (Clayton/AU), Brandon Sy, Julia Wong (Sydney/AU), David Powell, Adele Barugahare (Clayton/AU), Jai Tree (Sydney/AU), Marina Harper, John Boyce (Clayton/AU)
P 027 Posttranscriptional regulation in Escherichia coli probiotic strain under anaerobic conditions
Oleg Murashko, Sue Lin-Chao (Taipei/TW)
P 028 Uncovering and deciphering of new antisense RNAs in E. coli
Maxence Lejars, Céline Gracia, Rudi Zimmermann, Alexandre Maes (Paris/FR), Antonin Marchais (Zurich/CH), Mathieu Erhardt, Philippe Bertin (Strasbourg/FR), Fanette Fontaine, Maude Guillier, Eliane Hajnsdorf (Paris/FR)
P 029 Carbon source dependent antibiotic susceptibility in Pseudomonas aeruginosa through titration of Hfq by the regulatory RNA CrcZ.
Elisabeth Sonnleitner, Dorothea Heitzinger, Udo Bläsi (Vienna/AT)
P 030 Non-coding metatranscriptomics analysis of the animal gut microbiota in response to antibiotic treatment
Igor Morozov, Dorota Dobrzanska, Jess Rollason, Lauren Acton, Gareth Weedall (Liverpool/GB)
P 031 An mRNA degradation intermediate connects nutrients sensing with stress responses in Escherichia coli
Ira Alexandra Iosub, Stuart McKellar (Edinburgh/GB), Marta Marchioretto (Trento/IT), Karen Julia Nieken (Edinburgh/GB), Jai Tree (Sydney/AU), Gabriella Viero (Trento/IT), Sander Granneman (Edinburgh/GB)
P 032 Identification and characterization of a regulatory RNA, antisense of an IS481 transposase and the fimbriae serotype 2 genes in Bordetella pertussis
Alexandre D'Halluin, David Hot (Lille/FR)
P 033 The role of the RNA chaperone ProQ in the Gram negative bacterium Pasteurella multocida.
Emily Gulliver (Clayton/AU), Brandon Sy, Julia Wong (Sydney/AU), Deanna Deveson Lucas, Ralf B. Schittenhelm, David Powell (Clayton/AU), Jai Tree (Sydney/AU), Marina Harper, John Boyce (Clayton/AU)
P 034 CrcZ RNA cross-regulates Hfq-mediated oprD repression and leads to increased susceptibility to carbapenems
Petra Pusic, Elisabeth Sonnleitner, Udo Bläsi (Vienna/AT)
P 035 The CRISPR-Cas system of Mycobacterium tuberculosis
Malcolm White, Sabine Gruschow, Januka Anthukoralage, Tess Hoogeboom (St Andrews/GB)
P 036 Post-transcriptional gene regulation by an Hfq-independent sRNA in Caulobacter crescentus
Kathrin Fröhlich (Martinsried/DE, Princeton/US), Konrad Förstner (Würzburg/DE), Zemer Gitai (Princeton/US)
P 037 Regulation of Bacterial Gene Expression by Delivery of Antisense Oligonucleotides
Annika Schulz, Caroline Tawk, Jörg Vogel (Würzburg/DE)
P 038 Tandem Riboswitches Form Logic Gates to Control Ribonucleotide Biosynthesis and Signaling in Bacteria
Madeline E. Sherlock, Narasimhan Sudarsan, Shira Stav, Ronald Breaker (New Haven/US)
P 039 Development of a Bacterial Three-Hybrid Assay to probe sRNA-Chaperone Mechanisms
Smriti Pandey, Clara Wang, Kelly Chambers, Courtney Hegner, Katherine Berry (South Hadley, MA/US)
P 041 Concerted action of Hfq and Crc in Hfq-dependent translational regulation in Pseudomonas aeruginosa
Udo Bläsi, Elisabeth Sonnleitner (Vienna/AT), Xue-Yuan Pei, Ben F. Luisi (Cambridge/GB), Alexander Wulf, Henning Urlaub (Göttingen/DE), Sebastien Campagne, Frederic Allain (Zürich/CH)
P 042 Large scale identification of sRNA scr5239 target genes
Franziska Engel, Michael Vockenhuber, Beatrix Suess (Darmstadt/DE)
P 043 In vivo genome-wide probing of RNA structure in Bacillus subtilis reveals RNA regulatory features
Laura Ritchey, David Tack (University Park, University Park, PA/US), Sarah Assmann (University Park, PA/US), Philip Bevilacqua (University Park, PA, University Park/US), Paul Babitzke (University Park, PA/US)
P 044 Nitrogen-regulated antisense and trans-acting small RNAs identified through co-expression network analysis in the heterocystous cyanobacterium Nostoc sp. PCC 7120
Manuel Brenes-Álvarez, Ana Sousa-Ortega (Sevilla/ES), Jens Georg, Wolfgang R. Hess (Freiburg/DE), Agustín Vioque, Alicia M. Muro-Pastor (Sevilla/ES)
P 045 Heterocyst-specific (trans-acting and antisense) RNAs in the cyanobacterium Nostoc sp. PCC 7120
Elvira Olmedo-Verd, Manuel Brenes-Álvarez, Marina Minguet, Agustín Vioque, Alicia M. Muro-Pastor (Sevilla/ES)
20.03.2018 Please note that the posters from poster session 1 and 2 should be hung by Monday, 19:00 h and need to be removed by Wednesday, 09:00 h.
17:20–19:30
Room
Poster session 2
Torre del Oro
P 046 Ribosomal RNA methylation and ribosome maturation in the hyperthermophilic archaeon Nanoarchaeum equitans
Kenneth Seistrup, Simon Rose (Odense/DK), Ulf Birkedal, Henrik Nielsen (Copenhagen/DK), Harald Huber (Regensburg/DE), Stephen Douthwaite (Odense/DK)
P 047 Degradation of the bmrCD and yrzI operon mRNAs by the Ribosome-associated endonuclease 1 (Rae1) in Bacillus subtilis
Frederique Braun, Magali Leroy, Ciaran Condon (Paris/FR)
P 048 Isolation and characterization of some chlorpyriphos-degrading bacteria from different ecological niches of the province of ouargla in South-eastern of Algeria. 
Aloui Nabiha (Ghardaia/DZ)
P 049 Study of the sRNA content  in membrane vesicles from Pseudomonas aeruginosa PAO1 during  transition to stationary phase
Carla Perez Cruz, Ferran Briansó, Elena Mercade Gil (Barcelona/ES)
P 050 Identification of Hfq-binding RNAs in Caulobacter crescentus
Nadine de Assis, Rodolfo Ribeiro, Larissa da Silva, Alexandre Vicente (Sao Paulo/BR), Isabelle Hug, Urs Jenal (Basel/CH), Marilis Marques (Sao Paulo/BR)
P 051 Disruption of a large noncoding RNA-protein complex causes magnesium sensitivity
Kimberly Harris, Zhiyuan Zhou, Michelle Peters, Sarah Wilkins, Nicole Odzer, Ronald Breaker (New Haven/US)
P 052 Robust prediction of small RNA targets and evolutionary patterns of sRNA/target interactions
Patrick R. Wright, Martin Raden, Florian Eggenhofer, Stephan Klähn, Rolf Backofen, Wolfgang R. Hess, Jens Georg (Freiburg/DE)
P 053 Streptococcus suis small RNA rss04 contributes to the induction of meningitis by regulating capsule synthesis and by inducing biofilm formation in a mouse infection model
Zongfu Wu, Genhui Xiao, Huanyu Tang, Shouming Zhang, Chengping Lu (Nanjing/CN)
P 054 Expanding the targetome of Salmonella infection relevant small RNA PinT using MS2 affinity purification and RNA-seq (MAPS)
Sara Santos, Alexander Westermann, Jörg Vogel (Würzburg/DE)
P 055 Discovery of 224 novel structured RNA classes in bacteria using comparative genomics
Zasha Weinberg, Christina E. Weinberg (Leipzig/DE), Keith A. Corbino (New Haven/US), Tyler D. Ames (New York/US), James W. Nelson (Boston/US), Adam Roth, Kevin R. Perkins, Madeline E. Sherlock, Ronald Breaker (New Haven/US)
P 056 Discovery of RNA-protein complexes by Grad-seq for the opportunistic pathogens Enterococcus faecalis and E. faecium
Elisabeth Hansen, Charlotte Michaux, Silvia Di Giorgio, Lars Barquist, Jörg Vogel (Würzburg/DE)
P 057 RNA matchmakers regulating genes: exploring the RNA interactome of euryarchaeal Lsm & Hfq-like proteins
Robert Reichelt, Sarah Willkomm, Winfried Hausner, Dina Grohmann (Regensburg/DE)
P 058 Argonaute and CRISPR-Cas: a functional link for prokaryotic defense?
Sarah Willkomm, Dina Grohmann (Regensburg/DE)
P 059 Posttranscriptional regulation of SAM and m6A modification of RNA in the alpha-proteobacterium Sinorhizobium meliloti
Hendrik Melior (Giessen/DE), Konrad Förstner (Würzburg/DE), Christian Preußer, Jochen Bathke, Alexander Goesmann, Oliver Rossbach, Elena Evguenieva-Hackenberg (Giessen/DE)
P 060 Novel small RNAs identified by RIL-seq are encoded within different genomic elements
Liron Argaman, Amir Bar, Hanah Margalit (Jerusalem/IL)
P 061 One transcript, distinct stabilities: The methionine biosynthesis operon in Staphylococcus aureus
Freya D. R. Wencker, Sonja M. K. Schoenfelder, Wilma Ziebuhr (Würzburg/DE)
P 062 Identifying potential regulatory RNA drug targets in Streptococcus pneumoniae
Indu Warrier, Ariana Hazery, Nikhil Ram-Mohan, Zeyu Zhu, Jon S Anthony, Tim van Opijnen, Michelle M Meyer (Chestnut Hill/US)
P 063 Post-transcriptional inactivation of toxin-antitoxin systems via exonucleolytic degradation of protective stem-loops
Sara Masachis Gelo, Sandrine Chabas, Fanny Boissier (Bordeaux/FR), Hélène Arnion (Limoges/FR), Isabelle Iost, Fabien Darfeuille (Bordeaux/FR)
P 064 An RNA thermometer mediates translational control in Yersinia pseudotuberculosis
Christian Twittenhoff (Bochum/DE), Ann Kathrin Heroven, Petra Dersch (Braunschweig/DE), Franz Narberhaus (Bochum/DE)
P 065 Comparative multi-condition inter-strain transcriptomics identifies unexpected differences between global and African sequence types of Salmonella Typhimurium
Rocío Canals (Liverpool/GB), Disa Hammarlöf (Uppsala/SE), Carsten Kröger (Dublin/IE), Siân V. Owen, Wai Yee Fong, Will P. M. Rowe, Jay C. D. Hinton (Liverpool/GB)
P 066 A small RNA controls aminoglycosides resistance in E. coli
Sylvia Chareyre, Frederic Barras, Pierre Mandin (Marseille/FR)
P 067 Bacterial mRNA leader gives rise to trans-acting attenuator RNA and operating leader peptide
Hendrik Melior, Maximilian Stötzel, Siqi Li (Giessen/DE), Konrad Förstner (Würzburg/DE), Susanne Barth-Weber (Giessen/DE), Kathrin Baumgardt (Paris/FR), Elena Evguenieva-Hackenberg (Giessen/DE)
P 068 Maturation of rRNA in Helicobacter pylori: a cis-antisense RNA induces processing cleavages by RNase III
Isabelle Iost, Sandrine Chabas, Fabien Darfeuille (Bordeaux/FR)
P 069 Characterisation of a thiamine pyrophosphate riboswitch in Neisseria meningitidis
Francesco Righetti, Solange Materne, Edmund Loh (Solna (Stockholm)/SE)
P 070 SPIWE – a novel peptide encoded by a small RNA in Bacillus subtilis
Inam Ul Haq, Peter Müller, Sabine Brantl (Jena/DE)
P 071 The regulon of the RNA chaperone CspA and its auto-regulation in Staphylococcus aureus
Carlos J. Caballero, Pilar Menendez-Gil, Arancha Catalan-Moreno (Mutilva/ES), Marta Vergara-Irigaray, Begoña García (Mutilva, Pamplona/ES), Víctor Segura (Pamplona/ES), Naiara Irurzun, Maite Villanueva, Igor Ruiz de los Mozos (Mutilva/ES), Cristina Solano, Iñigo Lasa (Mutilva, Pamplona/ES), Alejandro Toledo-Arana (Mutilva/ES)
P 072 RecA-dependent tRNA cleavage is associated with activation of an RNA-repair operon in Salmonella Typhimurium
Kevin Hughes, Xinguo Chen, Sandra Wolin (Frederick/US)
P 073 Analysis of CsrA interactions with RNA targets in Vibrio cholerae
Alexandra Mey (Austin, Texas/US), Charles Midgett, Jon Kull (Hanover, New Hampshire/US), Shelley Payne (Austin, Texas/US)
P 074 VSR217, a novel conserved sRNA in Vibrio tasmaniensis is regulated by maltose and generated from the 5’ UTR of the malK gene
Xing Luo, Yiqin Deng, Philippe Bouloc, Annick Jacq (Orsay/FR)
P 075 Crucial roles of initiator tRNA in fidelity of translation initiation and ribosome maturation
Umesh Varshney, Sunil Shetty, Riyaz Shah (BANGALORE/IN)
P 076 Metabolic engineering of the purine pathway in Bacillus subtilis by interfering with PurR and riboswitch functions
Ahmed-Hocine Boumezbeur, Matthias Mack (Mannheim/DE)
P 077 Probing Fusobacterium nucleatum: a global RNA map for an emerging cancer-associated bacterium
Falk Ponath, Caroline Tawk, Jörg Vogel (Würzburg/DE)
P 078 Transcriptional regulation of the citric acid cycle mRNAs by the RoxS sRNA in Bacillus subtilis
Gergana Kostova, Ciaran Condon, Sylvain Durand (Paris/FR)
P 079 A prophage-encoded antisense RNA mediates phage superinfection exclusion in Salmonella Typhimurium
Siân V. Owen, Nicolas Wenner, Jay CD Hinton (Liverpool/GB)
P 080 Non-coding RNAs represent unanticipated species-specific players in Staphylococcus epidermidis biofilm control
Gabriella Marincola, Sonja M. K. Schoenfelder, Maike F. Lerch, Claudia Lange, Freya D. R. Wencker, Srinivasa Abishek Prakash, Konrad U. Förstner, Cynthia M. Sharma (Würzburg/DE), Kai M. Thormann (Gießen/DE), Susanne Engelmann (Braunschweig/DE), Wilma Ziebuhr (Würzburg/DE)
P 081 BpuR and SpoVG RNA-binding proteins regulate protein levels in Borrelia burgdorferi, the Lyme disease spirochete
Brian Stevenson, William Arnold, Kathryn Lethbridge, Christina Savage (Lexington, Kentucky/US)
P 082 Characterizing Riboswitches in Their Native Context
Arianne Babina, Ariana Hazery, Tim van Opijnen, Michelle Meyer (Chestnut Hill/US)
P 083 DrrS, a Mycobacterium tuberculosis Small RNA with a temporal twist
Kristine Arnvig, Alexandra Moores, Stefan Schwenk (London/GB), Simon Waddell (Brighton/GB)
P 084 Regulation of Acetate Metabolism: Coordination with the TCA Cycle via a Processed Small RNA
François De Mets (North Bethesda/US, Bruxelles/BE), Susan Gottesman (North Bethesda/US), Laurence Van Melderen (Bruxelles/BE)
P 085 Mechanisms of RNA processing-mediated post-transcriptional regulation of Methanolobus psychrophilus
Xiuzhu Dong, Lei Qi (Beijing/CN)
P 086 The Mn-specific yybP-ykpY orphan riboswitch regulates MgtA expression in Streptococcus pneumoniae
My Le, Julia E. Martin, Malcolm E. Winkler, David Giedroc (Bloomington/US)
P 087 RNA thermometers in transcripts of type 3 secretion system components in Yersinia pseudotuberculosis
Stephan Pienkoß, Marcel Holler, Franz Narberhaus (Bochum/DE)
P 088 The DPO-controlled VqmR sRNA regulates virulence gene expression in Vibrio cholerae
Roman Herzog, Nikolai Peschek, Kai Papenfort (Munich/DE)
P 089 Control of gene expression for restriction endonuclease in Type II Csp231I restriction-modification system.
Iwona Mruk, Monika Rezulak (Gdansk/PL)
P 090 Silencing of the shiga toxin Stx-1 in the Enterohemorrhagic Escherichia coli O157 (EHEC) via synthetic non-coding RNA; a novel antibacterial approach
Fazal Adnan, Samia Shafqat, Arslan Sarwar (Islamabad/PK)
21.03. 2018 Please note that the posters from poster session 3 and 4 should be hung by Wednesday, 11:00 h and need to be removed by Thursday, 11:00 h.
17:15–19:20
Room
Poster session 3
Andalucia
P 091 RIL-seq characterization of the sRNA-RNA interactomes of Hfq and ProQ: novel interplay among RNA chaperones
Philip Adams, Sahar Melamed, Aixia Zhang, Hongen Zhang, Gisela Storz (Bethesda/US)
P 092 Sigma 28/FliA Dependent Small RNAs Modulate Bacterial Flagella
Sahar Melamed, Joshua Mills, Aixia Zhang, Michal Jarnik, Gisela Storz (Bethesda/US)
P 093 Analysis of iCLIP data to identify targets of ribonucleases in Synechocystis sp. PCC 6803
Ute Hoffmann, Karsten Voigt, Olga Siadat, Juliane Behler, Annegret Wilde (Freiburg im Breisgau/DE)
P 094 Codon usage influences fitness through RNA toxicity in E. coli.
Pragya Mittal, James Brindle, Julie Stephen (Edinburgh/GB), Joshua B Plotkin (Philadelphia/US), Grzegorz Kudla (Edinburgh/GB)
P 095 Membrane localization keeps RNase E from interfering with early steps in ribosome assembly.
Lydia Hadjeras, Isabelle Canal, Marie Bouvier, Agamemnon Carpousis (Toulouse/FR)
P 096 CLIPseq guides the molecular characterization of CspC and CspE in Salmonella
Charlotte Michaux, Gianluca Matera, Lars Barquist, Erik Holmqvist, Jörg Vogel (Wuerzburg/DE)
P 097 A three-component system controlling small RNA GlmY expression in Escherichia coli
Yvonne Göpel, Boris Görke (Vienna/AT)
P 098 Role of the GlnZ small RNA at the interface between nitrogen and carbon metabolism in E. coli
Andrew Kouse, Gisela Storz (Bethesda/US)
P 099 Structural and functional characterization of an adaptor protein for a major endoribonuclease in Escherichia coli
Svetlana Durica-Mitic, Boris Görke (Vienna/AT)
P 100 Systematic identification of mRNA motifs associated with Hfq and class II mRNA-sRNA interactions
Amy Zheng, Andrew Santiago-Frangos, Subrata Panja, Sarah Woodson (Baltimore/US)
P 101 When antisense makes sense: exploring the role of RNA polymerase-binding RNA aptamers in control of bacterial antisense transcription
Andres Magan Garcia, Nadezda Sedlyarova, Fatinah El-Isa, Natascha Hartl, Renée Schroeder (Vienna/AT)
P 102 RIL-seq characterization of the sRNA-RNA interactomes of Hfq and ProQ: hierarchy of sRNAs bound by the RNA chaperones
Aixia Zhang, Sahar Melamed, Philip Adams, Hongen Zhang, Gisela Storz (Bethesda/US)
P 103 Mechanism and physiological significance of autogenous regulation of the Esherichia coli hfq gene
Teppei Morita, Hiroji Aiba (Suzuka, Mie/JP)
P 104 GLASSgo - Automated and reliable detection of sRNA homologs from a single input sequence
Steffen C. Lott (Freiburg/DE), Richard A. Schäfer (Stuttgart/DE), Martin Mann, Rolf Backofen, Wolfgang R. Hess (Freiburg/DE), Björn Voß (Stuttgart/DE), Jens Georg (Freiburg/DE)
P 105 DExD-box RNA helicase Lmo1722 of Listeria monocytogenes acts early in 50S ribosome biogenesis
Karolis Vaitkevicius, Jörgen Johansson (Umea/SE)
P 106 Identification, biosynthesis and decapping of NAD-capped RNAs in B. subtilis
Andres Jäschke, Jens Frindert, Yaqing Zhang, Gabriele Nübel, Masroor Kahloon, Leonie Kolmar (Heidelberg/DE)
P 107 Structural and Functional Analysis of Eukaryotic snoRNP Complexes Catalysing rRNA 2'-O-Ribose Methylations
Simone Hoefler, Petra Hinse, Teresa Carlomagno (Hannover/DE)
P 108 Small regulatory RNA C10 regulates antibiotic resistance in the plant pathogen Agrobacterium tumefaciens
Jessica Borgmann, Sina Schäkermann, Julia Bandow, Franz Narberhaus (Bochum/DE)
P 109 A transcriptomic study of the response to amino acid starvation in Escherichia coli using spike-in RNA for normalization
Bertil Gummesson, Shiraz Ali Shah, Michael A. Sørensen, Sine Lo Svenningsen (Copenhagen/DK)
P 110 Genome-wide identification of transcription start sites in Bacillus subtilis during fermentation
Line Dahl Poulsen, Adrian Geissler, Ferhat Alkan (Copenhagen/DK), Thomas Krogh Kallehauge, Anne Breüner (Bagsvaerd/DK), Christian Anthon, Jan Gorodkin, Jeppe Vinther (Copenhagen/DK)
P 111 The primary transcriptome, small RNAs, and regulation of antimicrobial resistance in Acinetobacter baumannii
Carsten Kröger (Dublin/IE), Keith D. MacKenzie (Regina/CA), Jay C. D. Hinton (Liverpool/GB), Karsten Hokamp (Dublin/IE), Andrew D. S. Cameron (Regina/CA)
P 112 Grad-seq analysis of giant bacteriophage ΦKZ infecting Pseudomonas aeruginosa
Laura Wicke, Cornelius Schneider, Jörg Vogel (Würzburg/DE)
P 113 The mechanism of glucosamine-6-phosphate sensing by the small RNA GlmY/GlmZ cascade in Escherichia coli
Muna Ayesha Khan, Yvonne Göpel (Vienna/AT), Ralf Heermann (Munich/DE), Boris Görke (Vienna/AT)
P 114 Staphylococcus aureus small RNAs driving fitness upon exposure to antibiotic targeting cell wall
Wenfeng Liu, Claire Morvan (Orsay Cedex/FR), Thao Nguyen Le Lam (Paris/FR), Philippe Bouloc (Orsay Cedex/FR)
P 115 Contrasting regulatory mechanisms of hdeD target mRNA by CyaR and RprA sRNAs
Karine Prévost, David Lalaouna, Eric Massé (Sherbrooke/CA)
P 116 Small non-coding RNAs and mutations in 16S ribosomal RNAs impact upon succinate utilization by Salmonella Typhimurium
Nicolas Wenner, Xiaojun Zhu, Jay C. D. Hinton (Liverpool/GB)
P 117 A new method based on paired end RNAseq analysis in order to identify small RNA
Simon Leonard, Florence Hommais, Sylvie Reverchon (Villeurbanne cedex/FR)
P 118 Multiple Small RNAs Post-transcriptionally Regulate Cyclopropane Fatty Acid Synthase
Colleen Bianco, Carin K. Vanderpool (Urbana/US)
P 119 Hfq-dependent Small RNA ArcZ regulates motility and exopolysaccharide production through the global regulator Lrp in Erwinia amylovora
Jeffrey Schachterle, George Sundin (East Lansing/US)
P 120 Global discovery of RNA-protein complexes in Clostridium difficile using Grad-seq
Franziska Faber (Braunschweig/DE), Jens Hör, Jörg Vogel (Würzburg/DE), Petra Dersch (Braunschweig/DE)
P 121 Shared and unique target regulation by two sigma E-controlled sRNAs in Vibrio cholerae
Nikolai Peschek, Roman Herzog, Kai Papenfort (Munich/DE)
P 122 The small RNA Teg41 Regulates Expression of the alpha Phenol Soluble Modulins and is Required for Virulence in Staphylococcus aureus
Rachel Zapf, Richard Wiemels, Rebecca Keogh, Emily Trzeciak, Kayla Howell, Andrew Caillet, Samantha Selhorst, Donald Holzschu (Athens/US), Jeffrey Bose (Kansas/US), Ronan Carroll (Athens/US)
P 123 Regulation of OmpA translation and Shigella virulence by an RNA thermometer
Erin Murphy (Athens/US), Johanna Roβmanith, Franz Narberhaus (Bochum/DE), Jacob Sieg, Jennifer Hines, Hebaallaha Hussein, Megan Fris (Athens/US)
P 124 Global searches for small RNAs in Bordetella pertussis using RNA-seq
Kyung Moon, Jeffers Nguyen, Charlotte Merzbacher, David Kim (Bethesda/US), Gloria Lee, Qing Chen, Scott Stibitz (Silver Spring/US), Deborah Hinton, Chin-Hsien Tai (Bethesda/US)
P 125 Supplementation of goat kids with coconut medium chain fatty acids in early life transiently influences the rumen methanogenic community and activity
Karen Goossens, Sieglinde Debruyne (Melle/BE), Alexis Ruiz Gonzales (Melle, Ghent/BE), Bart Ampe, Ellen De Keyser, Leen Vandaele (Melle/BE), Veerle Fievez (Ghent/BE)
P 126 Sequence Requirements for activity of the Escherichia coli Type I-E CRISPR-Cas system.
Lauren Cooper, Anne Stringer, Joseph Wade (Albany/US)
P 127 Rapid and extensive tRNA degradation occurs at the onset of amino acid starvation in E. coli
Sine Lo Svenningsen, Mette Kongstad, Thomas Søndergaard Stenum, Bertil Gummesson, Mathias Fessler, Ana J. Muñoz Gomez, Michael A. Sørensen (Copenhagen N/DK)
P 128 Single-cell microscopy reveals that levels of cyclic di-GMP vary significantly between Bacillus subtilis subpopulations
Cordelia Weiss, Wade C. Winkler (College Park/US)
P 129 Exploring a potential role for sRNAs during polyamine starvation
Nadim Majdalani, Manas Chattopadhyay, Christopher Keller, Herbert Tabor, Susan Gottesman (Bethesda/US)
P 130 A coordinated action of RNase III and RNase G controls enolase expression in response to oxygen availability in Escherichia coli
Minji Sim, Se-Hoon Sim, Kangseok Lee (Seoul/KR)
P 131 Preferential mRNA selection by heterogeneous rRNAs enables bacterial cells to adapt to environmental changes
Wooseok Song, Minju Joo, Ji-Hyun Yeom, Eunkyoung Shin, Minho Lee, Jeehyeon Bae, Kangseok Lee (Seoul/KR)
P 132 Mutually exclusive RNA secondary structures regulate translation initiation of DinQ in Escherichia coli
Knut Ivan Kristiansen, Ragnhild Weel-Sneve, James A. Booth, Magnar Bjørås (Oslo/NO)
P 133 A search for novel sRNAs in Staphylococcus aureus Newman highlights an unusual sRNA gene cluster with one sRNA interacting with the master riboregulator of quorum-sensing and virulence, RNAIII
Julie Bronsard, Gaetan Pascreau, Mohamed Sassi, Tony Mauro, Yoann Augagneur, Brice Felden (Rennes/FR)
P 134 The tRNA-linked repeats may represent a new class of small regulatory RNAs in E. coli.
Thomas Søndergaard Stenum (Copenhagen/DK), Erik Holmqvist (Uppsala/SE), Sine Lo Svenningsen, Michael A. Sørensen (Copenhagen/DK)
P 135 Hfq is not always essential for sRNA-mRNA pairing in Escherichia coli
David Lalaouna, Karine Prévost, Eric Massé (SHERBROOKE/CA)
21.03.2018 Please note that the posters from poster session 3 and 4 should be hung by Wednesday, 11:00 h and need to be removed by Thursday, 11:00 h.
17:15–19:20
Room
Poster session 4
Torre del Oro
P 136 Characterization of non-coding RNAs in the human pathogen Bordetella pertussis
Kristina Keidel (Prague 4/CZ), Fabian Amman (Vienna/AT), Ilona Bibova, Jan Capek, Jakub Drzmisek (Prague 4/CZ), David Hot (Lille/FR), Branislav Večerek (Prague 4/CZ)
P 137 The involvement of the antisense RNA RSaspufL in regulated formation of photosynthesis complexes in Rhodobacter sphaeroides
Carina M. Reuscher, Gabriele Klug (Giessen/DE)
P 138 Structural analysis of bacterial chaperon Hfq protein in complex with small RNAs
Ewelina Malecka-Grajek (Baltimore/US), Joon Ho Roh (Baltimore, College Park/US), Lixin Fan (Frederick/US), Sarah Woodson (Baltimore/US)
P 139 Adding players to the game: unveiling the complex regulation of the Opp ABC-transporter in Escherichia coli
Marie-Claude Carrier, David Lalaouna, Eric Massé (Sherbrooke/CA)
P 140 The small RNA RyhB regulates the expression of the cell division protein ZapB
Nadia Larabi, David Lalaouna, Eric Massé (Sherbrooke/CA)
P 141 GRADitude: A computational tool for the analysis of Grad-Seq data
Silvia Di Giorgio, Jens Hör, Jörg Vogel, Konrad U. Förstner (Würzburg/DE)
P 142 Deciphering the cross-talk between host and pathogen during intracellular persistence of Staphylococcus aureus within mast cells
Till Sauerwein (Würzburg/DE), Oliver Goldmann (Braunschweig/DE), Konrad Förstner (Würzburg/DE), Eva Medina (Braunschweig/DE)
P 143 Delivery of infection-specific RNAs to host cells by Salmonella Typhimurium outer membrane vesicles
Benedikt Beckmann, Senthil Subramanian (Berlin/DE)
P 144 Transcript autoregulation by 3'UTR-derived small RNAs in Vibrio cholerae
Mona Dotzler (Munich/DE), Konrad Förstner (Würzburg/DE), Kai Papenfort (Munich/DE)
P 145 Translation activation by mRNA secondary structures revealed by small RNA regulation
Jonathan Jagodnik, Claude Chiaruttini, Maude Guillier (Paris/FR)
P 146 Helping the host: roles of the small RNA DicF and small protein DicB of an E. coli cryptic prophage
Preethi T. Ragunathan, Carin K. Vanderpool (Urbana-Champaign/US)
P 147 crRNA maturation in Methanosarcina mazei Gö1 and the characterization of a novel archaeal lytic virus.
Andrea Ulbricht, Lisa Nickel, Katrin Weidenbach (Kiel/DE), Omer S. Alkhnbashi (Freiburg/DE), Anne Kupczok (Kiel/DE), Rolf Backofen (Freiburg/DE), Ruth Schmitz-Streit (Kiel/DE)
P 148 Reconstitution of the regulation of translation by small bacterial RNAs using in vitro translation assays
Joanna Mazur, Marcin Osuch, Marta Antoniewicz, Maria Ciesiolka, Zuzanna Wroblewska, Mikolaj Olejniczak (Poznan/PL)
P 149 Staphylococcus aureus S1 activates translation initiation of PSM toxins and stimulates the production of several other secreted virulence factors
Alessandra Marenna, Lucas Herrgott, Anne-Catherine Helfer, Javier Rol, Iskander Khusainov, Lauriane Kuhn, Pascale Romby, Isabelle Caldelari, Stefano Marzi (Strasbourg/FR)
P 150 Role of mRNA structure in the control of protein synthesis in E. coli
Zoe Chervontseva, Evgenia Khodzhaeva, Mikhail Gelfand, Andrey Mironov (Moscow/RU)
P 151 The mRNA sequence flanking sRNA binding sites affects the stability and the kinetics of sRNA binding to mRNA
Daria Sobanska, Elzbieta Kierzek, Ryszard Kierzek, Mikołaj Olejniczak (Poznan/PL)
P 152 Cell-wide purification of RNA-bound proteins in human and bacteria
Erika Urdaneta, Carlos Vieira, Matthias Selbach, Benedikt Beckmann (Berlin/DE)
P 153 The structure of Escherichia coli noncoding RNAs and their interactions with the chaperone protein ProQ
Ewa Stein, Adrian Sobusiak, Zuzanna Wróblewska, Mikołaj Olejniczak (Poznań/PL)
P 154 Unveiling the Hfq mediated RNA-RNA interactome of Salmonella Typhimurium
Gianluca Matera, Richa Bharti, Jörg Vogel (Würzburg/DE)
P 155 Small non-coding RNA-mediated control of virulence gene expression in Streptococcus pneumoniae
Jens Pettersen, Mikkel Girke Jørgensen (Odense C/DK)
P 156 Non-coding RNAs regulating cell cycle in Caulobacter crescentus
Wanassa Beroual (Marseille/FR), David Lalaouna (Sherbrooke/CA), Maude Guillier (Paris/FR), Eric Massé (Sherbrooke/CA), Emanuele Biondi (Marseille/FR)
P 157 Identification and characterization of pseudouridine in RNAs of the human pathogen Campylobacter jejuni
Elisabetta Fiore, Gaurav Dugar, Hock Siew Tan, Thorsten Bischler, Cynthia Sharma (Würzburg/DE)
P 158 Comparative RNA sequencing of the small RNA chaperon protein Hfq in Neisseria meningitidis
John Boss, Edmund Loh (Solna/SE)
P 159 Identification of novel RNA protein complexes in B. subtilis by Grad-Seq
Cornelius Schneider (Wuerzburg/DE), Alexander Reder (Greifswald/DE), Silvia Di Giorgio, Konrad Foerstner (Wuerzburg/DE), Uwe Voelker (Greifswald/DE), Joerg Vogel (Wuerzburg, Würzburg/DE)
P 160 The binding of Class II sRNAs to two different sites on matchmaker protein Hfq enables efficient competition for Hfq and annealing to regulated mRNAs
Joanna Mazur, Zuzanna Wróblewska, Agata Groszewska, Mikolaj Olejniczak (Poznań/PL)
P 161 An Hfq-binding sRNA controlling fatty acid metabolism in Vibrio cholerae
Michaela Huber, Jessica Radmer, Kai Papenfort (Munich/DE)
P 162 Does Ms1 sRNA regulate gene expression in Mycobacterium smegmatis?
Jarmila Hnilicova, Michaela Sikova, Martina Janouskova, Petra Palenikova, Jiri Pospisil, Olga Ramaniuk, Martin Prevorovsky, Libor Krasny (Prague/CZ)
P 163 Salmonella-host encounter: infection-relevant RNA-protein interactions
Yolanda Martínez Burgo, Petra Mailänder, Mikael Sellin, Erik Holmqvist (Uppsala/SE)
P 164 The α-proteobacterial trans-encoded small RNA MmgR: Regulation of polyhydroxybutyrate accumulation in Sinorhizobium meliloti
Antonio Jr. Lagares, Germán Ceizel Borella (Bernal/AR), Uwe Linne, Anke Becker (Marburg/DE), Claudio Valverde (Bernal/AR)
P 165 The conserved regulatory RNA RsaE down-regulates the arginine degradation pathway in Staphylococcus aureus
Tatiana Rochat (Jouy en Josas/FR), Chantal Bohn, Claire Morvan, Thao Nguyen Le Lam (Gif sur Yvette/FR), Fareha Razvi (Omaha/US), Claire Toffano-Nioche, Annick Jacq, Eric Jacquet (Gif sur Yvette/FR), Paul Fey (Omaha/US), Daniel Gautheret, Philippe Bouloc (Gif sur Yvette/FR)
P 166 Conserved putative RNA binding proteins regulate carbohydrate utilization in Bacteroides thetaiotaomicron
Amanda Adams, Zach A. Costliow, Carin K. Vanderpool, Patrick H. Degnan (Urbana/US)
P 167 One-step assembly of CRISPR arrays for multiplexed nucleic-acid cleavage and gene regulation
Chunyu Liao, Fani Ttofali, Rebecca Slotkowski, Steven Denny, Taylor D. Cecil, Ryan Leenay, Albert J. Keung, Chase L. Beisel (Raleigh/US)
P 168 Direct and Indirect Control of the Locus of Enterocyte Effacement Pathogenicity Island by Multiple Hfq-dependent sRNAs in enteropathogenic Escherichia coli
Shantanu Bhatt, Marisa Egan, Sarah Muche, Brian Critelli, Jamie Palmer, Valerie Jenkins, Elisabeth Mason, Jihad El-Fenej, Elizabeth Storm, Thomas Buerkert, Jasmine Ramirez, Christian Xander (Philadelphia/US)
P 169 The RNA Chaperone, Hfq, Represses the Locus of Enterocyte Effacement Pathogenicity Island in the emerging pathogen, Escherichia albertii
Marisa Egan, Jasmine Ramirez, Christian Xander, Shantanu Bhatt (King of Prussia/US)
P 170 A novel plasmid-transcribed regulatory sRNA, QfsR, controls chromosomal polycistronic mRNAs in Agrobacterium tumefaciens.
Benjamin Diel (Villeurbanne, Lyon/FR), Magali Dequivre (Lyon, Villeurbanne/FR), Camille Villard (Villeurbanne, Lyon/FR), Ludovic Vial (Lyon, Villeurbanne/FR), Florence Hommais (Villeurbanne, Lyon/FR)
P 171 ProQ, a major RNA binding protein that regulates motility, biofilm formation and prophage induction in Salmonella enterica serovar Typhimurium.
Youssef El Mouali, Jörg Vogel (Würzburg/DE)
P 172 Transcriptional landscape and regulatory roles of small non-coding RNAs in the oxidative stress response of Haloarchaea
Diego Gelsinger, Jocelyne DiRuggiero (Baltimore/US)
P 173 Streamlining Grad-seq with size exclusion chromatograhy
Jörg Vogel, Kotaro Chihara, Jens Hör (Wuerzburg/DE)
P 174 Systematic evaluation of computational and experimental methods for bacterial small RNA target identification
Alisa King, Carin K. Vanderpool, Patrick H. Degnan (Urbana/US)
P 175 Deciphering the mystery of Salmonella SraL sRNA
Inês Jesus Silva (Oeiras/PT), Alex Eyraud (Quebec/CA), Sandra Cristina Viegas (Oeiras/PT), David Lalaouna (Quebec/CA), Nara Figueroa-Bossi (Gif-sur-Yvette/FR)
P 176 The RNA-binding protein Hfq is a novel assembly factor in ribosome biogenesis
Ricardo F. dos Santos, José M. Andrade (Oeiras/PT), Irina Chelysheva, Zoya Ignatova (Hamburg/DE), Cecília M. Arraiano (Oeiras/PT)
P 177 YbeY: A new silencing enzyme that cleaves sRNA-mRNA hybrids
Margarida Saramago, Rute G. Matos (Oeiras/PT), Alexandra Peregrina, Marta Robledo, José I. Jiménez-Zurdo, Cecília M. Arraiano (Granada/ES)
P 178 Global identification of bacterial RNA-binding proteins by UV-crosslinking, phenol extraction, and mass spectrometry
Liis Andresen, Oskar Bergman, Erik Holmqvist (Uppsala/SE)
P 179 EF-P functions as a growth rate-dependent translation factor
Rodney Tollerson II, Anne Witzky, Michael Ibba (Columbus/US)
P 180 EF-P post-translational modification has variable impact on polyproline translation in Bacillus subtilis.
Anne Witzky, Katherine R. Hummels, Rodney Tollerson II, Andrei Rajkovic, Lisa A. Jones, Daniel B. Kearns, Michael Ibba (Columbus/US)
19:30–23:00
Room
Conference dinner
Restaurant